The San Francisco-based social media company said it expected expenses to increase by close to 20% in the fourth quarter compared with a year ago due to an increase in investments.
The company also cautioned that it was hard to predict how advertisers would react as the U.S. presidential election nears on Nov. 3.
Shares of Twitter fell to $44.00 in after-market trading.
Twitter said many companies paused ad spending during the second quarter due to widespread protests after the death of George Floyd in May and said there could be a similar dynamic with the U.S. election.
Twitter said it had 187 million monetizable daily active users (mDAU) during the third quarter, missing consensus analyst expectations of 195.2 million users, according to IBES data from Refinitiv. The figure stood at 186 million in the previous quarter.
Still, total revenue grew 14% year-over-year to $936 million during the quarter ended Sept. 30, beating analyst estimates of $777.15 million.
The growth was helped by updated advertising formats, improved ad measurement and the return of events that had been paused due to the pandemic, said Twitter Chief Financial Officer Ned Segal during an earnings call with analysts.
The company said outside of the election period, it expected revenue trends could continue or even improve in the current quarter.
Ad revenue in the third quarter grew 15% to $808 million from the same period a year ago, surpassing estimates of $645.95 million.
The company said it would delay the launch of a new advertising product until 2021 as it worked to integrate new mobile phone data privacy requirements.
Costs and expenses grew 13% from the same period last year to $880 million, as the company said it spent more on infrastructure-related expenses.
Net income in the third quarter was $28.66 million, or 4 cents per share, down from $36.5 million, or 5 cents per share in the year-ago quarter.